| dotpath

## Wiki

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Please help by correcting and extending the Wiki pages.

## Function

Draw a non-overlapping wordmatch dotplot of two sequences

## Description

dotpath generates a dotplot from two input sequences. The dotplot is an intuitive graphical representation of the regions of similarity between two sequences. Sequence "words" of a user-specified length are compared and all exact word matches between the two sequences are recorded. The set of the longest possible but non-overlapping matches is identified. The two sequences are the axes of the rectangular dotplot. Wherever there is an exact matching word in the two sequences a line is plotted.

## Algorithm

dotpath uses the same algorithm as diffseq and dottup for finding a minimal set of exact matches between two sequences. It finds all identical words of size `-wordsize` or greater in the two sequences. It then reduces the matches found to the minimal set of matches that do not overlap. This set is rendered as lines in the dotplot.

## Usage

Here is a sample session with dotpath

    % dotpath tembl:AF129756 tembl:BA000025 -word 20 -graph cps -overlaps
    Draw a non-overlapping wordmatch dotplot of two sequences

    Created dotpath.ps

Go to the input files for this example
Go to the output files for this example

## Command line arguments

    Draw a non-overlapping wordmatch dotplot of two sequences
    Version: EMBOSS:6.6.0.0

       Standard (Mandatory) qualifiers:
      [-asequence]         sequence   Sequence filename and optional format, or
                                      reference (input USA)
      [-bsequence]         sequence   Sequence filename and optional format, or
                                      reference (input USA)
       -wordsize           integer    [4] Word size (Integer 2 or more)
       -graph              graph      [$EMBOSS_GRAPHICS value, or x11] Graph type
                                      (ps, hpgl, hp7470, hp7580, meta, cps, x11,
                                      tek, tekt, none, data, xterm, png, gif, pdf,
                                      svg)

       Additional (Optional) qualifiers:
       -overlaps           boolean    [N] Displays the overlapping matches (in
                                      red) as well as the minimal set of
                                      non-overlapping matches
       -[no]boxit          boolean    [Y] Draw a box around dotplot

       Advanced (Unprompted) qualifiers: (none)
       Associated qualifiers:

       "-asequence" associated qualifiers
       -sbegin1            integer    Start of the sequence to be used
       -send1              integer    End of the sequence to be used
       -sreverse1          boolean    Reverse (if DNA)
       -sask1              boolean    Ask for begin/end/reverse
       -snucleotide1       boolean    Sequence is nucleotide
       -sprotein1          boolean    Sequence is protein
       -slower1            boolean    Make lower case
       -supper1            boolean    Make upper case
       -scircular1         boolean    Sequence is circular
       -squick1            boolean    Read id and sequence only
       -sformat1           string     Input sequence format
       -iquery1            string     Input query fields or ID list
       -ioffset1           integer    Input start position offset
       -sdbname1           string     Database name
       -sid1               string     Entryname
       -ufo1               string     UFO features
       -fformat1           string     Features format
       -fopenfile1         string     Features file name

       "-bsequence" associated qualifiers
       -sbegin2            integer    Start of the sequence to be used
       -send2              integer    End of the sequence to be used
       -sreverse2          boolean    Reverse (if DNA)
       -sask2              boolean    Ask for begin/end/reverse
       -snucleotide2       boolean    Sequence is nucleotide
       -sprotein2          boolean    Sequence is protein
       -slower2            boolean    Make lower case
       -supper2            boolean    Make upper case
       -scircular2         boolean    Sequence is circular
       -squick2            boolean    Read id and sequence only
       -sformat2           string     Input sequence format
       -iquery2            string     Input query fields or ID list
       -ioffset2           integer    Input start position offset
       -sdbname2           string     Database name
       -sid2               string     Entryname
       -ufo2               string     UFO features
       -fformat2           string     Features format
       -fopenfile2         string     Features file name

       "-graph" associated qualifiers
       -gprompt            boolean    Graph prompting
       -gdesc              string     Graph description
       -gtitle             string     Graph title
       -gsubtitle          string     Graph subtitle
       -gxtitle            string     Graph x axis title
       -gytitle            string     Graph y axis title
       -goutfile           string     Output file for non interactive displays
       -gdirectory         string     Output directory

       General qualifiers:
       -auto               boolean    Turn off prompts
       -stdout             boolean    Write first file to standard output
       -filter             boolean    Read first file from standard input, write
                                      first file to standard output
       -options            boolean    Prompt for standard and additional values
       -debug              boolean    Write debug output to program.dbg
       -verbose            boolean    Report some/full command line options
       -help               boolean    Report command line options and exit. More
                                      information on associated and general
                                      qualifiers can be found with -help -verbose
       -warning            boolean    Report warnings
       -error              boolean    Report errors
       -fatal              boolean    Report fatal errors
       -die                boolean    Report dying program messages
       -version            boolean    Report version number and exit

Qualifier | Type | Description | Allowed values | Default
--- | --- | --- | --- | ---
Standard (Mandatory) qualifiers
[-asequence] (Parameter 1) | sequence | Sequence filename and optional format, or reference (input USA) | Readable sequence | Required
[-bsequence] (Parameter 2) | sequence | Sequence filename and optional format, or reference (input USA) | Readable sequence | Required
-wordsize | integer | Word size | Integer 2 or more | 4
-graph | graph | Graph type | EMBOSS has a list of known devices, including ps, hpgl, hp7470, hp7580, meta, cps, x11, tek, tekt, none, data, xterm, png, gif, pdf, svg | EMBOSS_GRAPHICS value, or x11
Additional (Optional) qualifiers
-overlaps | boolean | Displays the overlapping matches (in red) as well as the minimal set of non-overlapping matches | Boolean value Yes/No | No
-[no]boxit | boolean | Draw a box around dotplot | Boolean value Yes/No | Yes
Advanced (Unprompted) qualifiers
(none)
Associated qualifiers
"-asequence" associated sequence qualifiers
-sbegin1 -sbegin_asequence | integer | Start of the sequence to be used | Any integer value | 0
-send1 -send_asequence | integer | End of the sequence to be used | Any integer value | 0
-sreverse1 -sreverse_asequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask1 -sask_asequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide1 -snucleotide_asequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein1 -sprotein_asequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower1 -slower_asequence | boolean | Make lower case | Boolean value Yes/No | N
-supper1 -supper_asequence | boolean | Make upper case | Boolean value Yes/No | N
-scircular1 -scircular_asequence | boolean | Sequence is circular | Boolean value Yes/No | N
-squick1 -squick_asequence | boolean | Read id and sequence only | Boolean value Yes/No | N
-sformat1 -sformat_asequence | string | Input sequence format | Any string |
-iquery1 -iquery_asequence | string | Input query fields or ID list | Any string |
-ioffset1 -ioffset_asequence | integer | Input start position offset | Any integer value | 0
-sdbname1 -sdbname_asequence | string | Database name | Any string |
-sid1 -sid_asequence | string | Entryname | Any string |
-ufo1 -ufo_asequence | string | UFO features | Any string |
-fformat1 -fformat_asequence | string | Features format | Any string |
-fopenfile1 -fopenfile_asequence | string | Features file name | Any string |
"-bsequence" associated sequence qualifiers
-sbegin2 -sbegin_bsequence | integer | Start of the sequence to be used | Any integer value | 0
-send2 -send_bsequence | integer | End of the sequence to be used | Any integer value | 0
-sreverse2 -sreverse_bsequence | boolean | Reverse (if DNA) | Boolean value Yes/No | N
-sask2 -sask_bsequence | boolean | Ask for begin/end/reverse | Boolean value Yes/No | N
-snucleotide2 -snucleotide_bsequence | boolean | Sequence is nucleotide | Boolean value Yes/No | N
-sprotein2 -sprotein_bsequence | boolean | Sequence is protein | Boolean value Yes/No | N
-slower2 -slower_bsequence | boolean | Make lower case | Boolean value Yes/No | N
-supper2 -supper_bsequence | boolean | Make upper case | Boolean value Yes/No | N
-scircular2 -scircular_bsequence | boolean | Sequence is circular | Boolean value Yes/No | N
-squick2 -squick_bsequence | boolean | Read id and sequence only | Boolean value Yes/No | N
-sformat2 -sformat_bsequence | string | Input sequence format | Any string |
-iquery2 -iquery_bsequence | string | Input query fields or ID list | Any string |
-ioffset2 -ioffset_bsequence | integer | Input start position offset | Any integer value | 0
-sdbname2 -sdbname_bsequence | string | Database name | Any string |
-sid2 -sid_bsequence | string | Entryname | Any string |
-ufo2 -ufo_bsequence | string | UFO features | Any string |
-fformat2 -fformat_bsequence | string | Features format | Any string |
-fopenfile2 -fopenfile_bsequence | string | Features file name | Any string |
"-graph" associated graph qualifiers
-gprompt | boolean | Graph prompting | Boolean value Yes/No | N
-gdesc | string | Graph description | Any string |
-gtitle | string | Graph title | Any string | Dotpath: $(asequence.usa) vs $(bsequence.usa)
-gsubtitle | string | Graph subtitle | Any string |
-gxtitle | string | Graph x axis title | Any string | $(bsequence.name)
-gytitle | string | Graph y axis title | Any string | $(asequence.name)
-goutfile | string | Output file for non interactive displays | Any string |
-gdirectory | string | Output directory | Any string |
General qualifiers
-auto | boolean | Turn off prompts | Boolean value Yes/No | N
-stdout | boolean | Write first file to standard output | Boolean value Yes/No | N
-filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N
-options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N
-debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N
-verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y
-help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N
-warning | boolean | Report warnings | Boolean value Yes/No | Y
-error | boolean | Report errors | Boolean value Yes/No | Y
-fatal | boolean | Report fatal errors | Boolean value Yes/No | Y
-die | boolean | Report dying program messages | Boolean value Yes/No | Y
-version | boolean | Report version number and exit | Boolean value Yes/No | N

## Input file format

### Input files for usage example

'tembl:AF129756' is a sequence entry in the example nucleic acid database 'tembl'

### Database entry: tembl:AF129756

    ID   AF129756; SV 1; linear; genomic DNA; STD; HUM; 184666 BP.
    XX
    AC   AF129756;
    XX
    DT   12-MAR-1999 (Rel. 59, Created)
    DT   14-NOV-2006 (Rel. 89, Last updated, Version 5)
    XX
    DE   Homo sapiens MSH55 gene, partial cds; and CLIC1, DDAH, G6b, G6c, G5b, G6d,
    DE   G6e, G6f, BAT5, G5b, CSK2B, BAT4, G4, Apo M, BAT3, BAT2, AIF-1, 1C7, LST-1,
    DE   LTB, TNF, and LTA genes, complete cds.
    XX
    KW   .
    XX
    OS   Homo sapiens (human)
    OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
    OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
    OC   Homo.
    XX
    RN   [1]
    RP   1-184666
    RX   DOI; 10.1101/gr.1736803.
    RX   PUBMED; 14656967.
    RA   Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D.,
    RA   Hood L.;
    RT   "Analysis of the gene-dense major histocompatibility complex class III
    RT   region and its comparison to mouse";
    RL   Genome Res. 13(12):2621-2636(2003).
    XX
    RN   [2]
    RP   1-184666
    RA   Rowen L., Madan A., Qin S., Shaffer T., James R., Ratcliffe A., Abbasi N.,
    RA   Dickhoff R., Loretz C., Madan A., Dors M., Young J., Lasky S., Hood L.;
    RT   "Sequence of the human major histocompatibility complex class III region";
    RL   Unpublished.
    XX
    RN   [3]
    RP   1-184666
    RA   Rowen L.;
    RT   ;
    RL   Submitted (22-FEB-1999) to the INSDC.
    RL   Department of Molecular Biotechnology, Box 357730 University of Washington,
    RL   Seattle, WA 98195, USA
    XX
    RN   [4]
    RP   1-184666
    RA   Rowen L.;
    RT   ;
    RL   Submitted (28-OCT-1999) to the INSDC.
    RL   Multimegabase Sequencing Center, University of Washington, PO Box 357730,
    RL   Seattle, WA 98195, USA

      [Part of this file has been deleted for brevity]

         aaaccagttt accaccactc ctaacactaa acttaaatct gactctaaat gtaagtccaa    181740
         tctgagccac aagcctaaag ttgaacttta tcctgcttta tgaattattc atccattcct    181800
         ccatttagtg agtatctgcg tgcctaacac atgctgggca ttgtcctaag gcaggaggga    181860
         catggaggca aagggatcag agaaggtacc agcacctgtg gagcttgtat tccagtgagg    181920
         ccagacggaa aagaaagaaa ctgaagaaga aattggtact atgagaaaat aagacaggct    181980
         gatgttgtaa gagtggcagg gagctacttt taaatacagt agtcagcaaa atcctctttg    182040
         agtgtttggg tggcactgga gctgagaccc aaatgacaaa aaatagtgac caggtaaaag    182100
         tttgggagca aagcatttca ggtaaaggga gcagctactg caaaggctgg aaggcggaac    182160
         caagctgggg gtgttgacga caaacagaag gccagtgtgg ctggagcaga gagagagact    182220
         gggaggcggg tgggagatga ggtcagagag gagggcaggg gccaggtcat gcagggccat    182280
         gcaagaaggg taaagcctct agatttcatc cagccacagg aagcctttaa aggtcgtcag    182340
         agtgtgtggt gcgtgcgtgt gtgtgtgtgt gtgtgtgtgt gttgcagggg agagaggggg    182400
         agggagagag agagagagag agagaagagg gaggtgagca gaggtgattg gatttttttt    182460
         tcttttgaca tggtgtcttg ctctgtggcc taggctggag tgcagtggca ccatcatagc    182520
         ccactgcaac ctcaaaacca tgggctcaag tcatccttcc acctcagctt cccaagtatc    182580
         taggactaca ggtgtgtgcc actgtgcctg gctaatttta aaaaatattt taaaattttt    182640
         gttgagacag ggtctatgct gctcaggctg gtctcgaact cctggtttca agtgatctgc    182700
         ccatcttggc ctcccaaagt ttttttttgt tagtttgaga ggcggtttcg ctcgttgccc    182760
         aggctggagt gcaatgactg atctcatctc actgcaacct ctgcctcctg ggttcaagcg    182820
         attctcctgc ttcagcctcc caagtagctg ggattacagg tgcatgccac cattcccggc    182880
         taattttttg tatttagtag agatggggtt tcaccatgtt agtcaggctg atctcaaact    182940
         cctgacctca ggtgatccgc ctgcctcagc ctcccaaagt tttgggatta caggtgtgag    183000
         ccaccatgct gggccagcct cccaaagttt tgggattaca ggcatgagtc accacactgg    183060
         ccctggattt tttttctttc ttttttttgg agacggagtc tcactctgtt gcccaggctg    183120
         gagtgcaatg gcgtaatctc agctcactgc aacctctgct gcccgggttc aaacgattct    183180
         cctgtcttag cctcctgagt agctgggatt ataggtgcat gccaccatgc ctggctaatt    183240
         tttgtacttt tagtagagaa agtacaccat cttggccagg ctggtctcga actcctgacc    183300
         tcaggtgatc cacttgcgtc ggcctcccaa agtgctggga ttacaggcgt gagacaccgc    183360
         acccagcctt tttttttttt tttcttttaa gacagaatcg ctctgtcacc caggctggag    183420
         tgcagtggca caatctcggc tcactgcaac ctctgcctcc caggtttaag caatccacct    183480
         atgtcagtct cccaagtagc tgggattata ggtgcatgtc accatgcctg gctaattttt    183540
         gtacttttag tatagaaagt acaccatgtt ggccaggctg gtcttgaact cctgacctca    183600
         agtgatccgc ctgcctcagc ctcccgaagt gctggaatta cagacatgtg ccactgcacc    183660
         cggcctggtt ttttttttct aagagatgga gtctcacttt tctgcccagg ttggagtgca    183720
         atggcaccat catagctcac tgcagccttc aactcttggc ctcaggcaat ccttgcacct    183780
         tagcctcgca gtgttgggat tacaggcatg agccactgag ccttgcctgg actttttttt    183840
         ttttttgaga tggcgtctcg ctctgttgcc caggttggag tgctacggca tgatcttggc    183900
         tcactgcaac ttccacctcc caggttcaag cgattctctt gcctcggccc cccgagtagc    183960
         tgggattaca ggcatgcgcc accgtgcctg gctaattttg gtatttttag tagagatagg    184020
         gtttcatcat gttgggcagg ctggtcttga actcctgacc tcgtgatcca cccacctcgg    184080
         cctcccaaag tgctgggatt ataggcatag ccaacgcgcc cagcctggac ttgtttttaa    184140
         aagatcactg tggctcctgt gtttaggctg gctggtagga gacaggtggc agtggcattg    184200
         atggtgaaga gaaaatagtg gcagccatgg agatggagag aagtagacaa gtttgggata    184260
         tattatacat tccaggggta gaaacaacag gactagatga tggattgatg ggtgggagat    184320
         gtagatactg ggagagaagc aggattctga tggatggaaa aactaaaaaa ttctattttg    184380
         ggtgtggtaa gtctaagtct attagacatg caagtagaga tgtcactggg cagatacaca    184440
         tctggatttc aggggcaagg tccaagctag agaaagaaac ctgggcatgg tcagcatgag    184500
         gatggtgttt aaagccatgg aacttatctt gtgcatccct ataagacccc tttgaggcac    184560
         ttgtttcccc tcacaatgga tgcagtgcat cttccattct gaattccaga ggcaacaacc    184620
         tcctgctcct agaagctaaa ctctccagac ttagtcttct gaattc                   184666
    //

### Database entry: tembl:BA000025

    ID   BA000025; SV 2; linear; genomic DNA; STD; HUM; 2229817 BP.
    XX
    AC   BA000025; AP000502-AP000521;
    XX
    DT   09-DEC-2004 (Rel. 82, Created)
    DT   17-JUN-2008 (Rel. 96, Last updated, Version 5)
    XX
    DE   Homo sapiens genomic DNA, chromosome 6p21.3, HLA Class I region.
    XX
    KW   .
    XX
    OS   Homo sapiens (human)
    OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
    OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
    OC   Homo.
    XX
    RN   [1]
    RP   1-2229817
    RA   Hirakawa M., Yamaguchi H., Imai K., Shimada J.;
    RT   ;
    RL   Submitted (21-AUG-2001) to the INSDC.
    RL   Mika Hirakawa, Japan Science and Technology Corporation (JST), Advanced
    RL   Databases Department; 5-3, Yonbancho, Chiyoda-ku, Tokyo 102-0081, Japan
    RL   (E-mail:mika@tokyo.jst.go.jp, URL:http://www-alis.tokyo.jst.go.jp/,
    RL   Tel:81-3-5214-8491, Fax:81-3-5214-8470)
    XX
    RN   [2]
    RA   Shiina S., Tamiya G., Oka A., Inoko H.;
    RT   "Homo sapiens 2,229,817bp genomic DNA of 6p21.3 HLA class I region";
    RL   Unpublished.
    XX
    DR   EPD; EP11158; HS_TNF.
    DR   EPD; EP11159; HS_LTA.
    DR   EPD; EP73522; HS_HLA-B.
    DR   EPD; EP73908; HS_GTF2H4.
    DR   EPD; EP73940; HS_NEU1.
    DR   EPD; EP74013; HS_VARS2.
    DR   EPD; EP74203; HS_MRPS18B.
    DR   EPD; EP74346; HS_HLA-E.
    DR   EPD; EP74389; HS_BAT1.
    DR   EPD; EP74485; HS_IER3.
    DR   Ensembl-Gn; ENSG00000096155; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000096171; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000111971; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000137310; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000137312; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000137313; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000137331; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000137332; Homo_sapiens.
    DR   Ensembl-Gn; ENSG00000137337; Homo_sapiens.

      [Part of this file has been deleted for brevity]

         ttggccccac cccagcatgt ctccaggttc ctctcagccc tggttccttt tggccctgca   2226900
         gtcacaatgg gcaacactgt gacgcaccct gtcctgtgtc acagtgtcat acactcaggc   2226960
         tcacattgcc cctaggccac ttgccagcca agggacatgg ccacattttg tgtcttctgc   2227020
         acctcagcct tgctttcaag tgcaggtgat gatggcaccc acgcagaaca aatgttattt   2227080
         gctatcttcg tcgagtttag tcatccaatt ttccaaccct cactgggcaa ggaagagtgt   2227140
         ggtttccacc aagaaggcag gatgtcagca gtcacagggg caaccaacag ggaaagccgc   2227200
         cggaaaatag accccacagg aagcacaggt gtccagtgga gatgggaacc ctgcagattt   2227260
         gaccgtcttt aagcagatta gagagattac cgttactaac aacttagcca taaaagttta   2227320
         ttagctattt tcaaaaagca taaaattatg taatataatt ttttttaaat ttccatcaat   2227380
         acaaaactaa tctgggcact gcaacttccg gtgggcaact gggataggcg gcatcatcag   2227440
         gaaggcgagc cctgccgtgc cccatgtgcc agtgccccag atggcggcag cctccccaga   2227500
         agcaccttgt atctcccctg cacagggcca gggtcccagc ttcccataca ccttctcctg   2227560
         ctttttcttt tctgtccttt cctttttcaa taaaccacct gcaaaaaggg aaaaccattc   2227620
         tgaggacaag aaacatgtca atgggaaata cacagttgcc agagggtaaa aggccctgtt   2227680
         cattctcatt gaaaagctca ggtatttctg ttaaagtctc tccttttact ttaggatgct   2227740
         gactcctgcg tccatctcaa cctgggcatc gtgccaccac cttcaagaag agaaaaacta   2227800
         agtagtgctt tgcaaagggg cagcagcatt tctcatttct gaccatgtca ggcacatggc   2227860
         catgcagatg agcaggtggg ggacacaggt gagtctccag acctgctctc ctcccacagt   2227920
         acattcttga gtctttttaa acagttgtga aaatgccaca gatgcaagca cctgtgggcc   2227980
         actcccatgg ggaccgttgc acaaggcagt gccactcatt ctcagaacct cctaccatgg   2228040
         gctatgctta gtgacccgag gccaagccaa ggaagacgcc agccacaggg tgccatcctc   2228100
         aggggcatgc tgccagcagg ggcaaagtta tccctagcaa caagatacag aaagaaagaa   2228160
         aaaaggaagg aaatgtagcc aatgggccgg ttcaggttct tgactttgcc acacaaaaga   2228220
         atttgagagc aagtccaaag taaaagtcag caagagaatt tattgcaaag tgaaagtaca   2228280
         ctctgacagc tgatcagagc agctgctcaa aagagagaca gtaccctccc ctcacgggag   2228340
         tcttacatga ttattcatga ataggtggga aggggtattg ttttaagcat gttctgtggt   2228400
         ctcttgaacg tgcatgcact gtggttgtac atatcagcac acacatctta cgtctcatta   2228460
         gcatcttaac ttccctctca gagttgtgtt tgctactatt gtaatgagca taggtcagcc   2228520
         caaggacact attcatgggt ttctgggctt cctcagatgt ggggatgcct cccttggctc   2228580
         ttctacctct ttgctgcagg atgttctaac cacaagccca ggatatggtt tgcgcactgt   2228640
         cgaacagctt gttctctcca tcaacctgac aagtctcttg tttcctttca agggaggctg   2228700
         tgaacaccct atctcactga cctcagaagg acagtacagc agtagccacc atgaccaaaa   2228760
         agatgattcc agaagtgcag gacaactccc tacccagagg ctgtggctgt gcagtaacac   2228820
         accaagaggg gagtccagct ggctctcagg gtgctcacta ccctcatctg ggggcctgga   2228880
         ggacgtcaat tcctgagaac gccacgttct agtgagtaga atgaactgag agatacacag   2228940
         caaagctcca catacttttc cttttctttg tgcccgcagt gttcttcatc agtgtgctct   2229000
         cgcttttcag ctactactgt tggctggctg gaaaaaatag aacaatagta aaaattagag   2229060
         accagtcttt ggtgatgaag agaaatattg gctacttcca gtattttcta gctttggtta   2229120
         tggttgcagt tttccagctc accttgtggg gatgaattca gaaaaaagtt acaaattgaa   2229180
         atgaacatgc cagaagtatt ggctcaaatc aacgttgtcc tattaagcca cttagtgaat   2229240
         caaaagaccg cttgttggac tgttaatctc ggtggccaga gaaaggagct gaagaaggtg   2229300
         ttgccagatc aggaacaaat aattacagcg gcaatagaaa atggaagacc acttgttcat   2229360
         aaccatttga ataagggcaa ggtgtatgga aacacattat gaactgatat tttcagtttt   2229420
         gtttgcaaga aaatgattaa taaggtgaaa taattgaagt atcacggaag atacattaaa   2229480
         aaaaaaaaaa gcctttgtac agtttgctgg agccacagat gtcctactcc agagcagaac   2229540
         aatgcctgaa tcttcagggt ccatttctgc cgcattcact agcaaccaca aatgtgactt   2229600
         aattttactt tggaaataat gcttacccat tgtgagatgc tgtaatatga accatcatta   2229660
         catgttaaca tggcacatgg aattttgagt gtctaagtta catttttaga gttgtttctt   2229720
         agtagccatg tgagtttcca ctccaaaaac acaagctaaa aacttgtttt gagtgaagga   2229780
         catctagggc aaatggtggc tgaaagtgaa tgagatc                            2229817
    //

## Output file format

In normal operation, a dotplot image is displayed.

With the `-data` qualifier a file of the positions of the matches in the minimal non-overlapping set of matches is output.

### Output files for usage example

### Graphics File: dotpath.ps

## Notes

For similar sequences, dotpath provides a convenient way to find a path that aligns the two sequences well. It is not a true optimal path as produced by the dynamic programming algorithms used in water or needle, but for very closely related sequences it will produce the same result. In contast to full alignment, it works very quickly with very long sequences.

The entire set of matches found can be displayed with the -overlaps qualifier. This shows all matches in red, except for those in the minimal path (non-overlapping set) which are shown in black, as normal.

Using a longer word size will create a dottplot with relatively less noise; the matches are longer and therefore more likely to have biological meaning. Such runs will be much faster, but of course are less sensitive.

## References

None

## Warnings

If you give a small word size with a very large sequence you will run out of memory. If this happens, try again with a larger word size.

## Diagnostic Error Messages

None

## Exit status

It always exits with status 0.

## Known bugs

None

## See also

Program name | Description
--- | ---
dotmatcher | Draw a threshold dotplot of two sequences
dottup | Display a wordmatch dotplot of two sequences
polydot | Draw dotplots for all-against-all comparison of a sequence set

This program is closely based on dottup with the addition of by default displaying only the minimal set of non-overlapping matches.

This program uses the same algorithm as diffseq for finding a minimal set of very good matches between two sequences. diffseq may be more convenient if you are looking at the differences between two nearly identical sequences.

## Author(s)

Gary Williams formerly at:
MRC Rosalind Franklin Centre for Genomics Research Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SB, UK

Please report all bugs to the EMBOSS bug team (emboss-bug © emboss.open-bio.org) not to the original author.

## History

Written 14 Aug 2000.

## Target users

This program is intended to be used by everyone and everything, from naive users to embedded scripts.

## Comments

None